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ORIGINAL ARTICLE
Year : 2018  |  Volume : 32  |  Issue : 2  |  Page : 140-143

Extended-spectrum beta-lactamase detection by extended-spectrum beta-lactamase-Nordmann/Dortet/Poirel test: Where time is the essence


Department of Microbiology, R.G. Kar Medical College, Kolkata, West Bengal, India

Correspondence Address:
Dr. Abhishek Sengupta
Department of Microbiology, R.G. Kar Medical College and Hospital, Kolkata - 700 037, West Bengal
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jms.jms_34_17

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Background: Extended-spectrum beta-lactamase (ESBL)-producing strains are causing an increasing problem worldwide. Infection control is most effective when ESBL strains are detected by rapid, sensitive, and specific tests and an early implementation of appropriate antibiotic therapy is initiated. Recently, a rapid and cost-effective biochemical test for the detection of ESBL, the ESBL Nordmann/Dortet/Poirel (NDP) test, has been implemented. Aims: The aim of the study was to detect ESBL by a rapid NDP test. Settings and Design: The prospective study was conducted in the Department of Microbiology at a tertiary care hospital. Materials and Methods: A total of 208 isolates were subjected to the ESBL NDP test. Simultaneously, these isolates were also tested for ESBL production by double-disc synergy test (DDST) method. The ESBL NDP test was also used directly with spiked blood cultures inoculated with ESBL producers and non-ESBL producers. Statistical Analysis Used: Descriptive statistics such as mean and ratio are used for the interpretation of the study. Results: Using the ESBL NDP test, 80 of 208 (40%) of the tested isolates produced ESBL, among which Escherichia coli were 56 (70%) and Klebsiella pneumonia were 24 (30%) which showed positive result. Our results, done directly on clinical isolates as well as on spiked blood cultures inoculated with them, showed a perfect correlation between the ESBL NDP test and DDST in detecting ESBLs. Conclusion: This inexpensive ESBL NDP test might be implemented worldwide, especially in resource-limited settings of developing countries to optimize rapid choices of antibiotics for treating severe infections and avoidance of overuse of carbapenems.


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