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Year : 2015  |  Volume : 29  |  Issue : 1  |  Page : 20-22

Acid phosphatase activity and sperm morphological changes in vaginal swab samples collected at various post coital intervals: A study

1 Department of Forensic Medicine, Regional Institute of Medical Sciences, Imphal, Manipur, India
2 Department of Biochemistry, Regional Institute of Medical Sciences, Imphal, Manipur, India

Correspondence Address:
Dr. Thounaojam Meera
Department of Forensic Medicine, Regional Institute of Medical Sciences, Imphal - 795 004, Manipur
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0972-4958.158924

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Background: In sexual assault investigation, the scientific proof of the alleged crime as well as determination of the time of assault is crucial. Materials and Methods: The study was carried out by analyzing 157 vaginal swab samples collected from women attending a tertiary health care teaching hospital, at Imphal and as well from the sexual assault cases, which were brought for medicolegal examination in the same hospital at different post coital intervals (PCIs). The samples were studied for sperm morphology and acid phosphatase (ACP) levels at different PCI were established. The findings were recorded and statistically analyzed. Results: Complete sperm was seen up to 16 hours and sperm heads were observed up to a PCI of 63 hours. The mean ACP level of >30 King-Armstrong Units (KAU) was observed in PCI of 1 day and >25 KAU in PCI of 2 days. The level of ACP showed more or less a gradual fall with respect to time with significant changes within 3 days. Conclusion: It may be concluded that an estimate of the timing of sexual assault may be given especially within 3 days of the assault, thereby emphasizing the importance of early reporting of such crimes.

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